High demand for millions of COVID-19 tests per day combined with a disrupted global supply chain has left many countries facing diagnostic shortages. In a recent Nature commentary, John Nkengasong, Director of the Africa Centres for Disease Control and Prevention, said, “the collapse of global cooperation [has] shoved Africa out of the diagnostics market....African countries have funds to pay for reagents but cannot buy them.”
Scientists across the world are therefore developing new tests that are faster, cheaper, adapted to needs of local health systems and easy to manufacture in order to overcome this challenge.
To enable scientists to access the research tools they need for their work, researchers from the Open Bioeconomy Lab at the University of Cambridge, the Lab de Tecnología Libre at iBio/PUC Chile and the FreeGenes Project at Stanford University teamed up with synthetic biology company Ginkgo Bioworks to design an open source toolkit that enables researchers to produce 16 of the most useful enzymes for a number of diagnostic techniques used to detect SARS-CoV-2, the virus which causes COVID-19.
“Designing the collections was a great collaborative effort between researchers with diverse expertise and different local needs for fighting the pandemic,” said Dr Chiara Gandini from Cambridge’s Department of Chemical Engineering and Biotechnology. “We designed it with other biologists in mind, making it as easy as possible for them to reconfigure the toolkit for their requirements.”
The ‘Molecular Diagnostic Toolkit’ comprises ready-to-use DNA to produce enzymes including DNA polymerases and reverse transcriptases – the enzymes used in gold standard RT-qPCR tests. These enzymes are also useful for tests like LAMP, which is faster and simpler than RT-qPCR and is rapidly being adopted by more labs. Control DNA is also included in the toolkit to validate that tests will specifically detect SARS-CoV-2 but not closely related viruses.
Image: Novel Coronavirus SARS-CoV-2
Credit: NIAID
Reproduced courtesy of the University of Cambridge