Scalable Weak Anion Exchange and Hydrophobic Interaction (AEX-HIC) Mixed-Mode Chromatography Resin Enables Small- to Large-Scale Purification for Research and Biotherapeutic Applications
HERCULES, Calif. — January 14, 2025 — Bio-Rad Laboratories, Inc., a global leader in life science research and clinical diagnostics products, announces the launch of Nuvia wPrime 2A Media, a scalable weak anion exchange and hydrophobic interaction (AEX-HIC) mixed-mode chromatography resin for small- to large-scale biomolecule purification. Bio-Rad's partners in the resin’s development successfully used the resin to remove host cell proteins, high and low molecular weight aggregates and impurities, and DNA from monoclonal and bispecific antibodies, and adeno-associated virus.
Built upon Bio-Rad’s Nuvia bead technology, Nuvia wPrime 2A Media features a polyacrylamide (acrylamide polymer) base bead that can be used for laboratory- and small-scale purification workflows, as well as for pilot-scale bioproduction and manufacturing-scale downstream processing. Bio-Rad’s Nuvia resins are designed to have minimal non-specific binding reactions, due to the hydrophilic nature of the bead polymer, and maintain a constant dynamic binding capacity over a wide range of flow rates. The Nuvia bead allows for fast mass transfer, is chemically and mechanically stable over a wide range of conditions and is easy to pack.
Developed in collaboration with Bio-Rad’s partners in industrial biotherapeutic production, Nuvia wPrime 2A Media provides a wide purification design space. The charge state of the resin’s functional ligand can be modulated by the pH of the buffer, enabling the purification of otherwise difficult-to-separate biomolecules from other impurities.
“Bio-Rad is committed to developing innovative chromatography resins for a wide variety of customer needs, and to advancing the efforts of researchers and therapy manufacturers alike in saving lives,” said Diab Elmashni, Director, Process Chromatography Business at Bio-Rad Laboratories. “As a leader in multimodal chromatography technology, Bio-Rad’s Nuvia and CHT™ Ceramic Hydroxyapatite families of resins and media are integral to pharmaceutical development and industrial-scale purification for many customers worldwide This new resin is unique to the market and addresses customer requests for a scalable, weak anion exchange and hydrophobic interaction mixed-mode resin.”
Visit bio-rad.com/wPrime2A to learn more about Nuvia wPrime 2A Media, and to request samples.
BIO-RAD and CHT are trademarks of BIO-RAD Laboratories, Inc. in certain jurisdictions.
